P2X3 RECEPTORS MEDIATE VISCERAL HYPERSENSITIVITY DURING ACUTE CHEMICALLY-INDUCED COLITIS AND IN THE POST-INFLAMMATORY PHASE VIA DIFFERENT MECHANISMS OF SENSITIZATION.

ObjectivesExperiments using P2X3 knock-out mice or more general P2X receptor antagonists suggest that P2X3 receptors contribute to visceral hypersensitivity.We aimed to investigate the effect of the selective P2X3 antagonist A-317491 on visceral sensitivity under physiological conditions, during acute colitis and in the post-inflammatory phase of c

Nur-Shirin Harun,1,2 Philippe Lachapelle,3,4 Jo Douglass2,31Department of Respiratory and Sleep Medicine, The Royal Melbourne Hospital, Melbourne, VIC, 3050, Australia; 2Lung Health Research Centre, The University of Melbourne, Melbourne, VIC, 3052, Australia; 3Department of Immunology and Allergy, The Royal Melbourne Hospital, Melbourne, VIC, 3050

The comet assay (also known as single-cell gel electrophoresis) is a technique for the detection of DNA damage at the level of the individual cell.It is a versatile, relatively simple to perform and sensitive method.Although Food and Snacks most investigations make use of its ability to measure DNA single-strand breaks, modifications to the method